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membranes  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank membranes
    Membranes, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/membranes/anti-Myosin+heavy+chain/pmc12828604-85-11-34
    Average 95 stars, based on 18 article reviews
    membranes - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: CRISPR gene editing in pluripotent stem cells reveals the function of MBNL proteins during human in vitro myogenesis
    Article Snippet: Protein extracts (15–20 μg) were loaded on a 4–12% sodium dodecyl sulphate–polyacrylamide gel electrophoresis gradient (NuPage Bis–Tris gels, Invitrogen®) and transferred onto Gel Transfer Stacks Nitrocellulose membranes (Invitrogen®) using the iBlot2 Dry Blotting System (Invitrogen®). .. Membranes were then incubated overnight at 4°C with the following primary antibodies: MBNL1–MB1a(4A8) (DSHB®, 1:1000), MBNL2–MB2a(3B4) (DSHB®, 1:1000), MBNL3–5A11(sc-136 168) (Santa Cruz, 1:100). .. After hybridization of the peroxidase-conjugated secondary antibody (1:10000), immunoreactive bands were revealed by using Amersham ECL Select Western Blotting Detection Reagents (GE Healthcare®).

    Article Title: Differential regulation of chloride homeostasis and GABAergic transmission in the thalamus
    Article Snippet: After separation, proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (BioRAD, Hercules, CA, USA) and blocked in TBST/5% milk (in mM; 24.8 Tris, 136.9 NaCl, 2.68 KCl, 0.1% Tween 20, and 5% nonfat dry milk, pH 7.5) for 2.5 h at room temperature. .. Membranes were divided at 75 kDa and incubation with the primary antibodies (rabbit-anti-KCC2, 1:5000, Merck-Millipore, Darmstadt, Germany (No. 07-432); mouse-anti-NKCC1 (T4), 1:2000, Developmental Studies Hybridoma Bank (DSHB), T4 was deposited to the DSHB by Lytle, C./Forbush III, B. (DSHB Hybridoma Product T4), Iowa City, IA, USA (No. AB 528406); rabbit-anti-β-tubulin, 1:5000, Covance, Princeton, NJ, USA (No. PRB-435P)) in TBST/1% milk was performed overnight at 4 °C with agitation. .. The secondary antibodies (goat-anti-rabbit HRP, 1:1000, Dako, Glostrup, Denmark (No. P0448) and goat anti-mouse HRP, 1:1000, Dako (No. P0447)) in TBST/1% milk were applied for 1 h at room temperature with agitation.

    Article Title: Loss of Glial Neurofascin155 Delays Developmental Synapse Elimination at the Neuromuscular Junction
    Article Snippet: .. Membranes were incubated with primary antibodies against -actin (rabbit, 1:1000 dilution, Abcam), pan-Nfasc (rabbit, 1:1000 dilution, Abcam), NF-H (rabbit, 1:10,000 dilution, Abcam), NF-M (mouse, dilution 1:2000 dilution, Developmental Studies Hybridoma Bank), and NF-L (rabbit, 1:3000 dilution, Millipore). ..

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: The fiber type of individual single fiber segments was determined using the dot blotting method previously described and now termed MyDoBID (Myosin heavy chain detection by Dot Blotting for IDentification of muscle fiber type)., Briefly, a polyvinylidene fluoride (PVDF) membrane was activated in 96% ethanol for 120 s and equilibrated in transfer buffer (Bio-Rad Laboratories Laboratories, Hercules, CA, USA) for 120 s. Following this, 1-μL (10%) of each fiber was spotted on activated PVDF membrane. .. After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). ..

    Article Title: Puromycin reveals a distinct conformation of neuronal ribosomes
    Article Snippet: Samples were run on 12% polyacrylamide gels, transferred onto 0.45μm nitrocellulose membranes [Bio Rad; 1620115] by wet transfer and blocked with 5% bovine serum albumin (BSA) [Sigma; A9647] in 1X Tris-Buffered Saline with Tween (TBS-T). .. Membranes were incubated with primary antibodies (anti-puromycin AB 2619605 Developmental Studies Hybridoma Bank) (1:1000) for 1 hour at room temperature, followed by HRP-conjugated secondary antibodies [ThermoFisher; #31430, #31460] and detected by chemiluminescence [Perkin Elmer; NEL105001EA]. .. In some cases, the blots were then stripped [ZmTech Scientific; S208070] and reblotted with an anti-S6 antibody [Cell Signaling #2217].

    Article Title: Resveratrol decreases inflammation and increases utrophin gene expression in the mdx mouse model of Duchenne muscular dystrophy.
    Article Snippet: q Work was performed at the University of Sou Exercise Science. qq Conference presentations: Southeast American Annual Meeting, Feb 2011.. Experimental Biology A Annual American College of Sports Medicine Me American College of Sports Medicine Annual Meeting * Corresponding author.. University of South Caro Science, Public Health Research Center, 3rd Floor, 92 SC 29208, USA.

    other:

    Article Title: Delivery and expression of mRNA therapeutics during ex vivo kidney perfusion: A feasibility study in porcine and human donor kidneys
    Article Snippet: Human EPO in OK cell cultures was quantified by an EPO DuoSet ELISA ( R&D Systems, DY286-05) according to the manufacturer’s protocols.

    Blocking Assay:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: The fiber type of individual single fiber segments was determined using the dot blotting method previously described and now termed MyDoBID (Myosin heavy chain detection by Dot Blotting for IDentification of muscle fiber type)., Briefly, a polyvinylidene fluoride (PVDF) membrane was activated in 96% ethanol for 120 s and equilibrated in transfer buffer (Bio-Rad Laboratories Laboratories, Hercules, CA, USA) for 120 s. Following this, 1-μL (10%) of each fiber was spotted on activated PVDF membrane. .. After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). ..

    Stripping Membranes:

    Article Title: Exercise attenuates stress-related signaling as sensed by higher phosphorylation of small heat shock proteins in skeletal muscle from older individuals
    Article Snippet: The fiber type of individual single fiber segments was determined using the dot blotting method previously described and now termed MyDoBID (Myosin heavy chain detection by Dot Blotting for IDentification of muscle fiber type)., Briefly, a polyvinylidene fluoride (PVDF) membrane was activated in 96% ethanol for 120 s and equilibrated in transfer buffer (Bio-Rad Laboratories Laboratories, Hercules, CA, USA) for 120 s. Following this, 1-μL (10%) of each fiber was spotted on activated PVDF membrane. .. After a series of steps involving drying, re-activating, washing, and blocking, membranes were incubated in antibodies (for full details, see Latchman et al., ): firstly, anti-myosin heavy chain IIa (MHCIIa) (mouse, monoclonal IgG, A4.74; Developmental Studies Hybridoma Bank (DSHB), Iowa city, IA, USA), followed by stripping and then anti-MHCI (mouse monoclonal IgM, A4.840; DSHB). ..



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    Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Journal: Journal of Sport and Health Science

    Article Title: Influence of diet-induced obesity and voluntary exercise training on cardiac lipids and mitochondrial function in mice

    doi: 10.1016/j.jshs.2025.101095

    Figure Lengend Snippet: Voluntary exercise alters left ventricle gene and protein expression related to mitochondrial biogenesis and dynamics in high fat- and chow-fed mice. (A) Western blot analysis to assess purity of crude mitochondria isolation where left ventricle mitochondrial pellet expresses mitochondrial marker TOM70 and COXIV and supernatant expresses calnexin. Protein expression of (B) LCLAT1 and (C) MFN2 in left ventricle-isolated crude mitochondria and (D) LCLAT1, (E) PGC-1α, and (F) MFN2 in left ventricle tissue from male VET or sedentary mice fed an HFD or chow diet. Left ventricle mRNA expression of (G) OPA1 and (H) DRP1 in male VET or sedentary mice fed an HFD or chow diet. Values were calculated relative to 18S housekeeper. Calnexin and β-actin were used as internal controls of protein loading in left ventricle samples and COXIV as internal control of protein loading in crude mitochondria samples. Analysis was performed using two-way analysis of variance with Tukey’s post hoc test for multiple comparisons. Data are expressed as mean ± standard error of the mean. (B–C) n : 5–6 per group. (D–F) n = 7 per group. (G and H) n = 9 per group. CE = chow exercise; CS = chow sedentary; COXIV = cytochrome c oxidase subunit 4; DRP1 = dynamin-related protein 1; HE = high fat diet exercise; HFD = high fat diet; HS = high fat diet sedentary; LCLAT1 = lysocardiolipin acyltransferase 1; MFN2 = mitofusin-2; OPA1 = optic atrophy 1; PGC-1α = peroxisome proliferator-activated receptor gamma coactivator-1α; TOM70 = translocase of outer mitochondria membrane 70; VET = voluntary exercise training.

    Article Snippet: Immunoblotting was performed with antibodies against lysocardiolipin acyltransferase 1(LCLAT1, PA5-25627; Thermo Fisher Scientific), PGC-1α (ab191838; Abcam, Cambridge, UK), MFN2 (PA5-118059; Thermo Fisher Scientific), ANP (sc-18811; Santa Cruz, Dallas, TX, USA), tumor necrosis factor alpha (TNFα, 3707; Cell Signaling, Danvers, MA, USA), phosphorylated adenosine monophosphate-activated protein kinase (AMPK, 2531; Cell Signaling), AMPKα (2532; Cell Signaling), Perilipin 5 (PA1-46215; Thermo Fisher Scientific), translocase of outer mitochondria membrane 70 (TOM70, 65675; Cell Signaling), cytochrome c oxidase subunit 4 (COXIV, 4844; Cell Signaling), and calnexin (208880; Merck, Rahway, NJ, USA).

    Techniques: Expressing, Western Blot, Isolation, Marker, Control, Membrane